فهرست:
فصل اول
کلیات صفحه
مقدمه 6
تاریخچه کشف هلیکوباکتر پیلوری 8
تاکسونومی طبقه بندی 11
1-2-1 گونه های هلیکوباکتر 13
1-2-1-1 هلیکو باکترهای معدی 14
1-2-1-2 هلیکو باکترهای روده ای 14
1-3 اختصاصات میکروبیولوژی هلیکو باکتر پیلوری 18
1-3-1 مورفولوژی 19
1-3-2 ساختمان ژنتیکی 20
1-3-3 فیزیولوژی و متابولیسم 21
1-3-4 ساختار دیواره سلولی 22
1-3-4-1 ساختار پپتیدو گلیکان 23
1-3-4-2 غشای خارجی 23
1-3-5 ساختار آنتی ژنیک 23
1-4 جایگاه هلیکو باکتر پیلوری 24
1-5 کلونیزاسیون هلیکو باکتر پیلوری 25
1-5-1-1 لکتین های (هماگلوتینین) سیالیک اسید 26
1-5-1-2 ادهزین های متصل شونده به Lewis b 27
1-5-1-3 آدهزین های متصل شونده به لیپید و سولفات 28
1-5-1-4 نقش لییپو پلی ساکارید در چسبندگی 28
1-5-1-5 نقش HSPs هلیکو باکتر پیلوری در چسبندگی 28
1-5-1-6 نقش OMPs در چسبندگی 28
1-5-1-5 نقش (neutrophil – activating protein) NapA در چسبندگی 29
1-6 فاکتورهای بیماریزا 31
1-6-1 اوره آز 31
1-6-2 لییپو پلی ساکارید و نقش آن در بیماریزای 32
1-6-3 پروتئین (cytotoxinn – associated gene) CagA 32
1-6-4 نقش CagA در سرطانزایی معدی 32
1-6-5 VacA (سیتوتوکسین واکوئله کننده) 33
1-6-6 IceA 34
1-6-7 نقش حرکت و شکل مارپیچی باکتری در بیماریزایی 34
1-6-8 سایر آنزیم های هلیکو باکتر پیلوری 35
1-6-8-1 کاتالاز 35
1-6-8-2 فسفولیپاز 35
1-6-8-3 پروتئاز 35
1-6-9 جذب آهن 36
1-7 مکانیسم بیماریزایی 37
1-8-ایمنی زایی توسط هلیکو باکتر پیلوری 40
1-9 تظاهرات بالینی 41
1-10 ارتباط بیماریهای مختلف هلیکو باکتر پیلوری 44
1-10-1 بیماری های عروق قلبی 44
1-10-2 کم خونی فقر آهن 44
1-10-3 سرطان حنجره 45
1-11 تشخیص 45
1-11-1 روش تهاجمی (Invasive) 46
1-11-2 غیر تهاجمی (Non-Invasive) 50
فصل دوم
مواد مورد نیاز و روش کار
2-1 جامعه مورد مطالعه 54
2-2 مواد مصرفی و روش انجام آزمایش 55
2-2-2 مراحل جمع آوری بیوپسی های معده 55
1-2-3 استخراج DNA از بیوپسی های معده 57
2-2-4 بررسی ژن های hpa A,babA2 ,hsp 70 با روش مولکولی PCR 59
فصل سوم
نتایج 66
فصل چهارم
بحث 75
نتیجه گیری 79
پیشنهادات 79
منابع 80
خلاصه انگلیسی
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